Ultrasound extraction of polysaccharides from guava leaves and their antioxidant and antiglycation activity. (October 2018)

• Record Type: Journal Article
• Authors: Luo, You; Peng, Bin; Liu, Yan; Wu, Yanan; Wu, Zhenqiang
• Is Part Of: Process biochemistry. Volume 73(2018)
• Page Start: 228
• Page End: 234
• Publication Date: 2018-10
• Subjects: Polysaccharides -- Guava leaves -- Ultrasonic-assisted extraction -- Antioxidant -- Antiglycation; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.08.003
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Ultrasonic conditions significantly affect extraction efficiency. Guava leaf polysaccharides (GLP) has good antioxidant activity. GLP exhibits remarkable hypoglycemic activity in vitro. Abstract: Plant polysaccharides possess multiple physiological activities. Box-Behnken design (BBD) was employed to optimize the ultrasound-assisted extraction of polysaccharides from guava leaves (GLP). It was found that ultrasonic time, temperature and power has a significant effect on the extraction efficiency. The yield of GLP was 1.00 ± 0.04% under the optimal condition of extraction time of 20 min, temperature of 62 ℃ and ultrasound power of 404 W. The DPPH and ABTS + radical scavenging rate of GLP was 56.38% and 51.73% at 100 μg/mL, respectively. The rate of inhibition of α-amylase by GLP was only 14.06% at 1 mg/mL; however, its α-glucosidase inhibition rate reached 99.54% at 100 μg/mL. These results suggested that the ultrasound-assisted extraction for GLP is effective and environmentally friendly and GLP has wide application potentials as natural antioxidants and α-glucosidase inhibitors.