Properties of partially denatured whey protein products: Formation and characterisation of structure. (January 2016)

• Record Type: Journal Article
• Authors: Zhang, Zhuo; Arrighi, Valeria; Campbell, Lydia; Lonchamp, Julien; Euston, Stephen R.
• Is Part Of: Food hydrocolloids. Volume 52(2016:Jan.)
• Page Start: 95
• Page End: 105
• Publication Date: 2016-01
• Subjects: Whey protein concentrate -- Microparticulated WPC -- Partially denatured WPC -- Scanning electron microscopy; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2015.06.009
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: Partially denatured whey protein (PDWPC) products have been manufactured using a controlled heating process that allows control of the degree of denaturation of the whey proteins. This is assessed by following the change in free sulphydryl content of the protein as heating progresses. This allows the formation of soluble whey protein aggregates of diverse particle size and morphology. The PDWPC's have been made using different manufacturing conditions (temperature, pH, degree of denaturation) to give aggregated PDWPC powders with a degree of denaturation in the range 45–98% and particle size 3–17 μm. Particle size analysis, scanning electron microscopy and density analysis show that the particles have aggregated structures that range from compact, particulate gel-like to fibrillar phase-separated structures, with intermediate structures formed under some conditions. These structures are consistent with the known gel structures formed in whey protein concentrate gels. The structure of the PDWPC particles differs from that of microparticulated whey proteins. The possibility of using PDWPC's as ingredients tailored to the needs of food manufacturers is discussed. Graphical abstract: Highlights: We present a method for the controlled manufacture of aggregated whey protein ingredients. Differing particle microstructure is produced through changing processing parameters. Structure of the protein particles is consistent with known gel structure formed by WPC.