Toxicity and oxidative stress induced by T-2 toxin and HT-2 toxin in broilers and broiler hepatocytes. (January 2016)

• Record Type: Journal Article
• Authors: Yang, Lingchen; Yu, Zezhong; Hou, Jiafa; Deng, Yifeng; Zhou, Zhenlei; Zhao, Zhiyong; Cui, Jun
• Is Part Of: Food and chemical toxicology. Volume 87(2016)
• Page Start: 128
• Page End: 137
• Publication Date: 2016-01
• Subjects: T-2 and HT-2 toxin -- Broilers -- Hepatocytes -- Toxicity -- Oxidative stress; T-2 T-2 toxin -- HT-2 HT-2 toxin -- GSH glutathione -- LDH lacate dehydrogenase -- ALT alanine aminotransferase -- AST aspartate aminotransferase -- ROS reactive oxygen species -- GSH-PX glutathione peroxidase -- MDA malondialdehyde -- CAT catalase -- FCRs feed conversion ratios -- TP total protein -- DMEM Dulbecco's modified Eagle's medium -- MTT thiazolyl blue tetrazolium bromide -- ALP alkaline phosphatase -- SOD superoxide dismutase -- HPLC–MS/MS high-performance liquid chromatography–tandem mass spectrometry -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- GST glutathione S-transferase -- PCR polymerase chain reaction -- D-HBSS D-Hank's balanced salt solution -- DMSO dimethyl sulfoxide -- DCFH-DA 2′, 7′-dichlorofluorescein diacetate -- TBA formation of thiobarbituric acid -- S.E standard error of measurement; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2015.12.003
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: T-2 and HT-2 toxins belong to mycotoxins which are found in human foods and animal chow. We investigated the toxicity and oxidative stress induced by T-2/HT-2 in broilers and chicken hepatocytes. Maize cultures of Fusarium poae was fed to broilers for 42 d, and the physiological index, biochemical index and expression of mRNAs related to oxidative stress were analyzed. Chicken hepatocytes were treated with different levels of T-2/HT-2, and the following parameters were detected: cell viability, GSH and MDA concentration, LDH leakage, activities of ALT/AST, ROS, GSH-PX, SOD and CAT, and expression of mRNA related to oxidative stress. In vivo, high levels of mycotoxins (4 mg/kg T-2 and 0.667 mg/kg HT-2) in feed caused significant reductions in body weight, weight gain, and serum total protein, and significant increases in feed conversion ratio, ALP, ALT/AST activities, and expression of mRNA related to oxidative stress. In vitro, cells treated with T-2/HT-2 showed reductions of GSH concentration and significant increases in LDH leakage, ALT/AST ROS, GSH-PX, SOD and CAT activities, MDA concentration, and expression of mRNA related to oxidative stress. Consequently, F. poae culture material and T-2/HT-2 induced toxicity and oxidative stress in vivo and in vitro, respectively. Highlights: This study revealed toxicity induced by T-2 and HT-2 toxin on broilers in vivo and in vitro. We obtained high levels of T-2/HT-2 from the maize inoculated with Fusarium poae . T-2 and HT-2 toxin caused toxicity and oxidative stress on broilers and chicken hepatocytes in a dose-dependent manner.