Hydroxyethyl cellulose-grafted loofa sponge-based metal affinity adsorbents for protein purification and enzyme immobilization. (November 2018)

• Record Type: Journal Article
• Authors: Lai, Wei-Jie; Lin, Sung-Chyr
• Is Part Of: Process biochemistry. Volume 74(2018)
• Page Start: 141
• Page End: 147
• Publication Date: 2018-11
• Subjects: Loofa sponge -- Immobilized metal affinity chromatography -- Protein purification -- Enzyme immobilization; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.08.024
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Loofa sponge-based IMAC adsorbents for protein purification was prepared. Grafting of hydroxyl ethylcellulose (HEC) increased the capacity of the adsorbents. Co(II)-loaded loofa sponge-HEC-IDA exhibited superior adsorption selectivity. Enzyme immobilized with the adsorbent retained 70% activity after 20 cycles. Abstract: The development of loofa sponge-based immobilized metal affinity adsorbents was reported in this study. We showed that by grafting hydroxyethyl cellulose (HEC) to loofa sponge the chelating capacity was increased by 117% from 2520 ± 45 μmole/g to 5478 ± 77 μmole/g. Results of equilibrium adsorption study showed that the adsorption capacity of the HEC-grafted loofa sponge-based immobilized metal affinity adsorbent for the recombinant His-tagged trehalose synthase, 2.45 mg/g, was 62% higher than that of the adsorbent without HEC conjugation, 1.51 mg/g. The employment of the trehalose synthase-loaded loofa sponge-based immobilized metal affinity adsorbent as the biocatalyst for the conversion of maltose to trehalose in repeated-batch operations was conducted. It was found that after 20 cycles 70% of the initial activity was retained. The decline in activity could be attributed predominantly to the desorption of trehalose synthase from the loofa sponge-based immobilized metal affinity adsorbent.