A novel method for the extraction of prodigiosin from bacterial fermenter integrated with sequential batch extraction reactor using magnetic iron oxide. Issue 10 (October 2016)

• Record Type: Journal Article
• Authors: K.V., Arivizhivendhan; M., Mahesh; R., Boopathy; R., Regina Mary; G., Sekaran
• Is Part Of: Process biochemistry. Volume 51:Issue 10(2016:Oct.)
• Page Start: 1731
• Page End: 1737
• Publication Date: 2016-10
• Subjects: Prodigiosin -- Functionalized iron oxide -- Sequential batch reactor -- Solvent free extraction -- Bacterial secondary metabolite; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.07.012
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Schematic diagram of prodigiosin extraction using [Fe3O4]F from fermented medium. Highlights: The manuscript focused on the preparation of robust [Fe3 O4 ]F for the extraction of prodigiosin from fermented medium. A novel reactor fabrication and processing step was evaluated for the extraction of prodigiosin. About 95% of solvent usage was reduced in the proposed method. SBER was effectively extracting the prodigiosin from the continuous fermentation unit. Abstract: A novel method for the extraction of prodigiosin from bacterial fermentation was achieved using an integrated sequential batch extraction reactor (SBER) containing functionalized iron oxides ([Fe3 O4 ]F ). The [Fe3 O4 ]F was prepared using diethanolamine to impart quaternary amine group onto Fe3 O4 . The quaternary amine group imparted to Fe3 O4 enhanced the prodigiosin adsorption efficiency. Two SBER were integrated with the continuous fermenter for the continuous extraction of prodigiosin. The present investigation established a method to conserve solvent by about 95% during the prodigiosin extraction step. The proposed extraction method can be regarded as solvent free direct extraction of bacterial bioactive compounds from bacterial fermented medium.