A mitochondria-targetable fluorescent probe for detection of bisulfite in living cells. Issue 31 (1st August 2018)

• Record Type: Journal Article
• Authors: Guo, Xiao; Zhu, Wei-Jin; Wei, Xue-Rui; Xu, Yu-Jie; Sun, Ru; Ge, Jian-Feng
• Is Part Of: Analytical methods. Volume 10:Issue 31(2018)
• Page Start: 3872
• Page End: 3877
• Publication Date: 2018-08-01
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay01543k
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A colorimetric fluorescent probe based on benzo[ g ]indolium was prepared as a mitochondria-targeted probe for detecting bisulfite. Abstract : A new colorimetric semi-cyanine fluorescent probe (3 ) based on benzo[ g ]indolium was designed and prepared. Probe3 exhibited a clear OFF–ON fluorescence response at 460 nm toward HSO3 − (0–8.0 eq.) within a short response time (3 min). In addition, probe3 had good selectivity and competitiveness, with excellent sensitivity and a low detection limit (4.4 nM). The detection mechanism relied on nucleophilic HSO3 − attacking the CC double bond in a 1, 4-nucleophilic addition reaction. Importantly, the confocal fluorescence results indicated that probe3 targeted mitochondria and could detect HSO3 − in living cells.