A mitochondria-targetable fluorescent probe for detection of bisulfite in living cells. Issue 31 (1st August 2018) - Guo, Xiao
Abstract : A colorimetric fluorescent probe based on benzo[ g ]indolium was prepared as a mitochondria-targeted probe for detecting bisulfite. Abstract : A new colorimetric semi-cyanine fluorescent probe (3 ) based on benzo[ g ]indolium was designed and prepared. Probe3 exhibited a clear OFF–ON fluorescence response at 460 nm toward HSO3 − (0–8.0 eq.) within a short response time (3 min). In addition, probe3 had good selectivity and competitiveness, with excellent sensitivity and a low detection limit (4.4 nM). The detection mechanism relied on nucleophilic HSO3 − attacking the CC double bond in a 1, 4-nucleophilic addition reaction. Importantly, the confocal fluorescence results indicated that probe3 targeted mitochondria and could detect HSO3 − in living cells.