Improving succinic acid production by Actinobacillus succinogenes from raw industrial carob pods. (October 2016)

• Record Type: Journal Article
• Authors: Carvalho, Margarida; Roca, Christophe; Reis, Maria A.M.
• Is Part Of: Bioresource technology. Volume 218(2016)
• Page Start: 491
• Page End: 497
• Publication Date: 2016-10
• Subjects: Succinic acid -- Actinobacillus succinogenes -- Carob pods -- Fed-batch fermentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.06.140
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Water extracts containing 92 g/L sugars can be obtained from raw carob pods. A volumetric productivity of 1.67 g SA/L.h was obtained during batch cultivation. By-products formation was reduced using fed-batch mode, yielding 0.94 g SA/g sugars. Carob pods are an excellent substrate for A. succinogenes and succinic acid production. Abstract: Carob pods are an inexpensive by-product of locust bean gum industry that can be used as renewable feedstock for bio-based succinic acid. Here, for the first time, unprocessed raw carob pods were used to extract a highly enriched sugar solution, afterwards used as substrate to produce succinic acid using Actinobacillus succinogenes . Batch fermentations containing 30 g/L sugars resulted in a production rate of 1.67 g SA/L.h and a yield of 0.39 g SA/g sugars. Taking advantage of A. succinogenes ' metabolism, uncoupling cell growth from succinic acid production, a fed-batch mode was implemented to increase succinic acid yield and reduce by-products formation. This strategy resulted in a succinic acid yield of 0.94 g SA/g sugars, the highest yield reported in the literature for fed-batch and continuous experiments, while maintaining by-products at residual values. Results demonstrate that raw carob pods are a highly efficient feedstock for bio-based succinic acid production.