Inactivation of Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 on cantaloupes by octenidine dihydrochloride. (September 2016)

• Record Type: Journal Article
• Authors: Upadhyay, Abhinav; Chen, Chi-hung; Yin, Hsinbai; Upadhyaya, Indu; Fancher, Samantha; Liu, Yanyan; Nair, Meera Surendran; Jankelunas, Leanne; Patel, Jitendra R.; Venkitanarayanan, Kumar
• Is Part Of: Food microbiology. Volume 58(2016:Sep.)
• Page Start: 121
• Page End: 127
• Publication Date: 2016-09
• Subjects: Octenidine hydrochloride -- Chitosan -- Cantaloupes -- Listeria monocytogenes -- Escherichia coli O157:H7 -- Salmonella spp; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2016.04.007
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: The efficacy of a new generation disinfectant, octenidine dihydrochloride (OH), as wash and coating treatments for reducing Listeria monocytogenes (LM), Salmonella spp. (SAL), and Escherichia coli O157:H7 (EC) on cantaloupe was investigated. Cantaloupe rind plugs inoculated separately with the three bacterial species (∼8 log CFU/cm 2 ) were washed for 1, 3, 5 min at 25 °C in water, or chlorine (200 ppm), ethanol (1%), OH (0.01, 0.05, 0.1%) and surviving populations were measured after treatment. Additionally, inoculated cantaloupe rind plugs were coated with 2% chitosan or chitosan containing OH (0.01, 0.05, 0.1%) and sampled for surviving pathogens. Subsequently, the antimicrobial efficacy of OH wash and coating (0.1, 0.2%) on whole cantaloupes was determined. All OH wash reduced LM, SAL, and EC on cantaloupe rinds by > 5 log CFU/cm 2 by 2 min, and reduced populations to undetectable levels (below 2 log CFU/cm 2 ) by 5 min (P < 0.05). Similarly, OH coating on cantaloupe rinds reduced the pathogens by 3–5 log /cm 2 (P < 0.05). Washing and coating whole cantaloupes with OH reduced the three pathogens by at least 5 log and 2 log CFU/cm 2, respectively (P < 0.05). Results suggest that OH could be used as antimicrobial wash and coating to reduce LM, SAL, and EC on cantaloupes. Highlights: Octenidine wash (0.1, 0.2%) decreased pathogen populations by at least 5 log CFU/cm 2 on whole cantaloupes (P < 0.05). Octenidine coating reduced Listeria and Salmonella by >5 log CFU/cm 2 (P < 0.05). Octenidine coating reduced Escherichia coli O157:H7 by >2 log CFU/cm 2 (P < 0.05).