Cationic beta-lactoglobulin nanoparticles as a bioavailability enhancer: Effect of surface properties and size on the transport and delivery in vitro. (1st August 2016)

• Record Type: Journal Article
• Authors: Teng, Zi; Luo, Yangchao; Li, Ying; Wang, Qin
• Is Part Of: Food chemistry. Volume 204(2016)
• Page Start: 391
• Page End: 399
• Publication Date: 2016-08-01
• Subjects: BLG (bovine) beta-lactoglobulin -- CBLG cationic beta-lactoglobulins. These include C-EDA and C-P600 in this study -- EDA ethylenediamine -- PEI (branched) polyethyleneimine -- EDC N-(3-dimethylaminopropyl)-N-ethylcarbodiimide -- C-EDA ethylene-derived cationic beta-lactoglobulin -- C-P600 polyethyleneimine (MW 600)-derived cationic beta-lactoglobulin -- PBS phosphate buffer saline -- HBSS Hank's balanced salt solution -- DMEM Dulbecco's modified Eagle's medium -- FBS fetal bovine serum -- BSA bovine serum albumin -- FITC fluorescein isothiocyanate -- PSM porcine stomach mucin -- TEER transepithelial electrical resistance -- MTT assay 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay -- DLS dynamic light scattering -- QCM quartz crystal microbalance -- GI tract gastrointestinal tract -- SGF simulated gastric fluid -- SIF simulated intestinal fluid; Beta-lactoglobulin (BLG) -- Cationic polymers -- Surface properties -- Nanoparticles -- Encapsulation -- Delivery systems -- Mucoadhesion; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.02.139
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Cationic beta-lactoglobulin nanoparticles have been developed as novel nutraceutical/drug carriers. Satisfactory results were observed in simulated physiological processes ranging from digestion to cellular uptake. Surface properties and sizes of the nanocarriers determined their performances in different biological processes. Abstract: BLG (beta-lactoglobulin) and CBLG (cationic BLG developed by our lab) were evaluated as potential nutraceutical/drug carriers. The cationic corona conferred CBLG with superior integrity and drug retention under gastrointestinal conditions, at most 40-fold higher mucoadhesion, up to 30-fold greater transepithelial permeation and, at most 285% higher cellular uptake, compared to BLG. Furthermore, the more hydrophilic CBLG species exhibited better mucoadhesion, while the more hydrophobic one exhibited higher cellular uptake. Intriguingly, protein molecules were more cytotoxic and exhibited up to 175% higher tight junction-opening capacity than did protein nanoparticles, whereas the nanoparticles displayed up to 770% higher mucoadhesion, greater transepithelial permeation and elevated cellular uptake. Finally, all these surface properties and performances were significantly altered as CBLG bound to serum proteins. Possible mechanisms underlying these findings are discussed in detail. This research sheds some light on the development of protein-based nanoencapsulants and their performance upon oral administration.