Vitamins C and E (ascorbate/α-tocopherol) provide synergistic neuroprotection in the jejunum in experimental diabetes. Issue 4 (December 2015)

• Record Type: Journal Article
• Authors: Tashima, Cristiano Massao; Hermes-Uliana, Catchia; Perles, Juliana Vanessa Colombo Martins; de Miranda Neto, Marcilio Hubner; Zanoni, Jacqueline Nelisis
• Is Part Of: Pathophysiology. Volume 22:Issue 4(2015)
• Page Start: 241
• Page End: 248
• Publication Date: 2015-12
• Subjects: Antioxidant -- Diabetes mellitus -- Enteric nervous system -- Neuropathy -- Oxidative stress -- Supplementation; Physiology, Pathological -- Periodicals; 571.9
• DOI: 10.1016/j.pathophys.2015.09.003
• Languages: English
• ISSNs: 0928-4680

Abstract

Abstract: The present study evaluated the synergistic effects of the association of ascorbic acid and α-tocopherol on myenteric in the jejunum of diabetic rats. The rats were randomly divided into four equal groups: untreated normoglycemic (UC), untreated diabetic (UD), ascorbic acid and α-tocopherol-treated normoglycemic (CAE) and ascorbic acid and α-tocopherol-treated diabetic (DAE). The rats from the CAE and DAE group received supplementation with ascorbic acid (1 g/L in water) and α-tocopherol (1% in chow). At 210-days-old, the animals were sacrified and their jejunum was collected and submitted to immunohistochemistry. Quantitative and/or morphometric analysis were performed. Supplementation with ascorbic acid and α-tocopherol prevented the cell loss of myenteric neurons expressing HuC/D and TrkA in an equivalent proportion. We also observed a reduction of the CGRP nerve fiber varicosities and the prevention of the increased cell body size of submucosal VIP neurons ( p < 0.05). The association of ascorbic acid and α-tocopherol reduced the deleterious effects of diabetes promoting protection on the enteric neurons.