Genetic characterization of strains of Saccharomyces uvarum from New Zealand wineries. (April 2015)

• Record Type: Journal Article
• Authors: Zhang, Hanyao; Richards, Keith D.; Wilson, Sandra; Lee, Soon A.; Sheehan, Hester; Roncoroni, Miguel; Gardner, Richard C.
• Is Part Of: Food microbiology. Volume 46(2015:Apr.)
• Page Start: 92
• Page End: 99
• Publication Date: 2015-04
• Subjects: Saccharomyces uvarum -- Saccharomyces eubayanus -- Microsatellite polymorphism -- Genome sequence -- Sulfite tolerance -- Cold fermentation; PCR polymerase chain reaction -- YPD yeast extract peptone dextrose -- SGM synthetic grape medium -- ITS internal transcribed spacer -- EDTA ethylenediaminetetraacetic acid; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2014.07.016
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: We present a genetic characterization of 65 isolates of Saccharomyces uvarum isolated from wineries in New Zealand, along with the complete nucleotide sequence of a single sulfite-tolerant isolate. The genome of the New Zealand isolate averaged 99.85% nucleotide identity to CBS7001, the previously sequenced strain of S. uvarum . However, three genomic segments (37–87 kb) showed 10% nucleotide divergence from CBS7001 but 99% identity to Saccharomyces eubayanus . We conclude that these three segments appear to have been introgressed from that species. The nucleotide sequence of the internal transcribed spacer (ITS) region from other New Zealand isolates were also very similar to that of CBS7001, and hybrids showed complete genetic compatibility for some strains, with tetrads giving four viable progeny that showed 2:2 segregations of marker genes. Some strains showed high tolerance to sulfite, with genetic analysis indicating linkage of this trait to the transcription factor FZF1, but not to SSU1, the sulfite efflux pump that it regulates in order to confer sulfite tolerance in Saccharomyces cerevisiae . The fermentation characteristics of selected strains of S. uvarum showed exceptionally good cold fermentation characteristics, superior to the best commercially available strains of S. cerevisiae . Highlights: New Zealand isolates of Saccharomyces uvarum are genetically compatible with CBS7001. A sequenced isolate of S. uvarum has three introgressed regions from Saccharomyces eubayanus . Sulfite tolerance in S. uvarum requires FZF1. Microsatellite repeats in S. uvarum show low levels of polymorphism. Some New Zealand isolates of S. uvarum are highly cryotolerant.