Antimicrobial activity of gallic acid against thermophilic Campylobacter is strain specific and associated with a loss of calcium ions. (April 2015)

• Record Type: Journal Article
• Authors: Sarjit, Amreeta; Wang, Yi; Dykes, Gary A.
• Is Part Of: Food microbiology. Volume 46(2015:Apr.)
• Page Start: 227
• Page End: 233
• Publication Date: 2015-04
• Subjects: Antimicrobial -- Calcium loss -- Campylobacter coli -- Campylobacter jejuni -- Gallic acid; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2014.08.002
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Gallic acid has been suggested as a potential antimicrobial for the control of Campylobacter but its effectiveness is poorly studied. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of gallic acid against Campylobacter jejuni ( n = 8) and Campylobacter coli ( n = 4) strains was determined. Gallic acid inhibited the growth of five C. jejuni strains and three C. coli strains (MIC: 15.63–250 μg mL −1 ). Gallic acid was only bactericidal to two C. coli strains (MBC: 125 and 62.5 μg mL −1 ). The mechanism of the bactericidal effect against these two strains (and selected non-susceptible controls) was investigated by determining decimal reduction times and by monitoring the loss of cellular content and calcium ions, and changes in cell morphology. Gallic acid did not result in a loss of cellular content or morphological changes in the susceptible strains as compared to the controls. Gallic acid resulted in a loss of calcium ions (0.58–1.53 μg mL −1 and 0.54–1.17 μg mL −1, respectively, over a 180 min period) from the susceptible strains but not the controls. Gallic acid is unlikely to be an effective antimicrobial against Campylobacter in a practical sense unless further interventions to ensure an effective bactericidal mode of action against all strains are developed. Highlights: We investigated the antimicrobial action of gallic acid against Campylobacter . Gallic acid inhibited the growth of 5/8 Campylobacter jejuni and 3/4 Campylobacter coli strains. Gallic acid was only bactericidal against 2 of the C. coli . The bactericidal mode of action was associated with calcium ion loss. Gallic acid alone is unlikely to be effect for control of Campylobacter .