Assessment of new environmental quorum quenching bacteria as a solution for membrane biofouling. (October 2017)

• Record Type: Journal Article
• Authors: Yavuztürk Gül, Bahar; Koyuncu, Ismail
• Is Part Of: Process biochemistry. Volume 61(2017)
• Page Start: 137
• Page End: 146
• Publication Date: 2017-10
• Subjects: QS quorum sensing -- QQ quorum quenching -- AHL acyl-homoserine lactone -- AIP autoinducing peptides -- AI-2 autoinducer-2 -- MBR membrane bioreactor -- EPS extracellular polymeric substances -- TMP trans-membrane pressure -- C8-HSL N-octanoyl-homoserine lactone -- LB Luria–Bertani medium; Membrane bioreactor -- Quorum quenching -- N-Acyl-homoserine lactone -- AHL degradation -- Quorum sensing; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.05.030
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: 19 new QQ bacteria were isolated from environmental samples. T5 and T6 completely degraded 200 nM AHL within 30 and 60 s in aqueous solution. QQ fiber was developed as a new immobilization medium. TMP values of control and QQ MBR showed that QQ-fiber with T5 is an effective biofouling control agent with 25% efficiency. Abstract: This study evaluated quorum quenching (QQ), which exists among bacteria that are isolated from saltern, pond and marine habitats, and applied selected QQ bacterium to inhibit biofilm formation in the membrane bioreactor (MBR). We identified nine genera belonging to 19 N -octanoyl-homoserine lactone (C8-HSL)-degrading bacteria, namely Shewanella, Acinetobacter, Klebsiella, Bacillus, Deftia, Vibrio, Comamonas, Microbacterium, and Pseudomonas . Both Bacillus sp. T5 and Delftia lacustris T6 were isolated from a saltern and degraded all the C8-HSL; thus, demonstrating the highest QQ capability. T5 was immobilized in a new immobilization medium named QQ-fiber, which was composed of floating 20-cm hollow fibers. To enlighten the antibiofouling potential of newly isolated Bacillus sp. T5, it was applied to an MBR and the QQ effect was monitored during 12 days of operation. According to transmembrane pressure (TMP) values, QQ bacterium led to less biofouling than the control reactor. In MBRs operated with T5, TMP was decreased with 25% efficiency. The decrease in biofouling resulted from QQ-fiber activity, and it was seen that T5 could be used successfully to inhibit biofilm formation in MBR.