Hyperoside promotes ex vivo expansion of hematopoietic stem/progenitor cells derived from cord blood by reducing intracellular ROS level. (September 2018)

• Record Type: Journal Article
• Authors: Zhang, Wenyan; Zhang, Weiwei; Zhang, Xu; Lu, Qin; Cai, Haibo; Tan, Wen-Song
• Is Part Of: Process biochemistry. Volume 72(2018)
• Page Start: 143
• Page End: 151
• Publication Date: 2018-09
• Subjects: CD34+ cells -- Reactive oxygen species level -- Ex vivo expansion -- Hyperoside -- Hematopoietic stem/progenitor cells; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.06.016
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Hyperoside promoted ex vivo expansion of CD34 + cells and CD3 + CD38 − cells. Hyperoside favored better biological function of expanded CD34 + cells. Hyperoside reduced intracellular ROS level and percentages of apoptotic cells. Abstract: Intracellular reactive oxygen species (ROS) accumulation during culture process compromise cord blood-derived hematopoietic stem/progenitor cells (CB-HSPCs) proliferation potential dramatically. In this work, the effects of hyperoside (Hyp) on CD34 + cells were investigated by the analysis of cell expansion and secondary expansion potential, with the objective to optimize the culture conditions for ex vivo expansion of HSPCs. The results showed that the expansion of total cells treated with 1 μM Hyp reached 54.9 ± 9.6 folds, which was significantly higher than that of control cells (42.0 ± 8.1 folds, P < 0.05), however, the percentages of CD34 + cells and CD34 + CD38 − cells were similar in two cultures, leading to significantly higher expansion of CD34 + cells and CD34 + CD38 − cells in Hyp-treated cultures ( P < 0.05). Importantly, it was found that CD34 + cells cultured with Hyp possessed better secondary expansion ability and colony forming potential, which meant better self-renewal potential. Furthermore, the addition of 1 μM Hyp reduced the ROS level in total cells, CD34 + cells and CD34 + CD38 − cells, and protected cells from apoptosis ( P < 0.05). Together, Hyp is beneficial for the ex vivo expansion of functional HSPCs, which can be possibly related to its capability of downregulating the intracellular level of ROS.