6‐Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP‐1‐Derived Macrophages. Issue 14 (25th June 2018)

• Record Type: Journal Article
• Authors: Wang, Dongdong; Hiebl, Verena; Ladurner, Angela; Latkolik, Simone L.; Bucar, Franz; Heiß, Elke H.; Dirsch, Verena M.; Atanasov, Atanas G.
• Is Part Of: Molecular nutrition & food research. Volume 62:Issue 14(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-06-25
• Subjects: atherosclerosis -- ATP‐binding cassette transporter A1 -- ATP‐binding cassette transporter G1 -- cardiovascular diseases -- cholesterol efflux; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201800011
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Ginger is reported to be used for the prevention and treatment of cardiovascular diseases (CVD). Cholesterol efflux from macrophage foam cells is an important process in reverse cholesterol transport, whose increase may help to prevent or treat CVD. In this study, we investigated the effects of 6‐dihydroparadol from ginger on macrophage cholesterol efflux. Methods and results: We show that 6‐dihydroparadol concentration‐dependently enhances both apolipoprotein A1‐ and human plasma–mediated cholesterol efflux from cholesterol‐loaded THP‐1‐derived macrophages using macrophage cholesterol efflux assay. 6‐Dihydroparadol increases protein levels of both ATP‐binding cassette transporters A1 and G1 (ATP‐binding cassette transporter A1 [ABCA1] and ATP‐binding cassette transporter G1 [ABCG1]) according to Western blot analysis. The ABCA1 inhibitor probucol completely abolishes 6‐dihydroparadol‐enhanced cholesterol efflux. Furthermore, increased ABCA1 protein levels in the presence of 6‐dihydroparadol were associated with both increased ABCA1 mRNA levels and increased ABCA1 protein stability. Enhanced ABCG1 protein levels were only associated with increased protein stability. Increased ABCA1 protein stability appeared to be the result of a reduced proteasomal degradation of the transporter in the presence of 6‐dihydroparadol. Conclusion: We identified 6‐dihydroparadol from ginger as a novel promoter of cholesterol efflux from macrophages that increases both ABCA1 and ABCG1 protein abundance. This newly identified bioactivity might contribute to the antiatherogenic effects of ginger. Abstract : 6‐Dihydroparadol, which is present in ginger, enhances both apolipoprotein A1‐ and human plasma‐mediated cholesterol efflux from cholesterol‐loaded macrophages. 6‐Dihydroparadol increases protein levels of both ATP‐binding cassette transporter A1 (ABCA1) and ATP‐binding cassette transporter G1 (ABCG1). Increased ABCA1 protein levels in the presence of 6‐dihydroparadol are associated with both increased ABCA1 mRNA levels and increased ABCA1 protein stability. Increased ABCG1 protein levels are only associated with increased protein stability.