An innovative method for immobilizing sucrose isomerase on ε-poly-l-lysine modified mesoporous TiO2. (15th November 2015)

• Record Type: Journal Article
• Authors: Wu, Lingtian; Liu, Yi; Chi, Bo; Xu, Zheng; Feng, Xiaohai; Li, Sha; Xu, Hong
• Is Part Of: Food chemistry. Volume 187(2015)
• Page Start: 182
• Page End: 188
• Publication Date: 2015-11-15
• Subjects: SIase sucrose isomerase -- EPL ε-poly-l-lysine -- M-TiO2 mesoporous titanium dioxide -- EPL-M-TiO2 ε-poly-l-lysine-functionalized mesoporous titanium dioxide -- SI-M-TiO2 sucrose isomerase immobilized on mesoporous titanium dioxide -- SI-EPL-M-TiO2 sucrose isomerase immobilized on ε-poly-l-lysine-functionalized mesoporous titanium dioxide; Titanium dioxide (PubChem CID: 26042) -- Sucrose (PubChem CID: 5988) -- Isomaltulose (PubChem CID: 439559) -- Trehalulose (PubChem CID: 162104); M-TiO2 -- Sucrose isomerase -- ε-Poly-l-lysine -- Isomaltulose -- Immobilization; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.04.072
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: M-TiO2 was modified by ε-poly-l -lysine successfully. ε-Poly-l -lysine played an important role in increasing enzymes loading on carriers. Immobilized SIase showed superior activity, stability and longer half-life. Generation of isomaltulose from sucrose using immobilized SIase. Abstract: Sucrose isomerase (SIase) is the key enzyme in the enzymatic synthesis of isomaltulose. Mesoporous titanium dioxide (M-TiO2 ) and ε-poly-l -lysine-functionalized M-TiO2 (EPL-M-TiO2 ) were prepared as carriers for immobilizing SIase. SIase was effectively immobilized on EPL-M-TiO2 (SI-EPL-M-TiO2 ) with an enzyme activity of 39.41 U/g, and the enzymatic activity recovery rate up to 93.26%. The optimal pH and temperature of immobilized SIase were 6.0 and 30 °C, respectively. SI-EPL-M-TiO2 was more stable in pH and thermal tests than SIase immobilized on M-TiO2 and free SIase. Km of SI-EPL-M-TiO2 was 204.92 mmol/L, and v max was 45.7 μmol/L/s. Batch catalysis reaction of sucrose by SI-EPL-M-TiO2 was performed under the optimal conditions. The half-life period of SI-EPL-M-TiO2 under continuous reaction was 114 h, and the conversion rate of sucrose after 16 batches consistently remained at around 95%, which indicates that SI-EPL-M-TiO2 has good operational stability. Thus, SI-EPL-M-TiO2 can be used as a biocatalyst in food industries.