Fermentation optimization and mathematical modeling of glycerol-based microbial poly(3-hydroxybutyrate) production. (August 2018)

• Record Type: Journal Article
• Authors: Das, Mriganko; Grover, Abhinav
• Is Part Of: Process biochemistry. Volume 71(2018)
• Page Start: 1
• Page End: 11
• Publication Date: 2018-08
• Subjects: Poly(3-hydroxybutyrate) (PHB) -- Fermentation -- Mathematical model -- Kinetics -- Production; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.05.017
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Batch statistical and mechanical models for the Poly-3-hydroxybutyrate (PHB) production were developed. A kinetic model was extended to fed-batch for enhanced PHB production. The proposed model helped in designing various nutrients feeding regimes. Cultivation at constant feeding showed a 5.4 fold higher PHBV concentration. Abstract: Poly(3-hydroxybutyrate) (PHB) belongs to a class of polyhydroxyalkanoates that are synthesized by prokaryotic microbes and serve as intracellular carbon and energy storage compounds. PHB-based plastics offer several advantages over conventional petroleum-based synthetic plastics, like biodegradability, renewability and low carbon footprints among others, thus promulgating a biobased market from niche to high volume applications. Considerable industrial interests exist in PHB; their cost of production is a major limiting factor. The present work focuses on the development of an economically viable glycerol-based method for PHB production, drawing on statistical and mechanistic models. An enhanced growth-associated PHB content of 40% (wt/wt) and cell biomass of 1.35 g/L were obtained at a glycerol concentration of 40 g/L in the model guided constant fed-batch strategy. This study facilitates an alternative bioprocess for sustainable production of high-value bioplastics.