A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo. Issue 11 (14th May 2018)

• Record Type: Journal Article
• Authors: Wang, Xiaoyi; Min, Jie; Wang, Weijie; Wang, Yan; Yin, Gui; Wang, Ruiyong
• Is Part Of: Analyst. Volume 143:Issue 11(2018)
• Page Start: 2641
• Page End: 2647
• Publication Date: 2018-05-14
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an00586a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A porphyrin-based highly selective sensor for hypochlorite was applied in real-time detection of exogenous and endogenous ClO − in biological systems. Abstract : Reactive oxygen species (ROS), especially HOCl/ClO −, have been demonstrated to play essential roles in both physiological and pathological processes, and an abnormal level of HOCl/ClO − is related to some diseases. In this work, a very fast responsive (within 30 seconds) porphyrin-based fluorescent probe, TPP-TCF, for ClO − with a NIR emissive wavelength was prepared. This probe exhibited excellent selectivity towards ClO − and would not be interfered with by other ROS and typical nucleophiles. The limit of detection (LOD) for ClO − was evaluated to be 0.29 μM, indicating high sensitivity towards ClO − . In further bioimaging experiments, TPP-TCF displayed low-cytotoxicity and good cell penetrability for recognizing exogenous ClO − in HeLa cells. Moreover, this probe was successfully applied in imaging endogenous ClO − in living animals.