A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo. Issue 11 (14th May 2018) - Wang, Xiaoyi
Abstract : A porphyrin-based highly selective sensor for hypochlorite was applied in real-time detection of exogenous and endogenous ClO − in biological systems. Abstract : Reactive oxygen species (ROS), especially HOCl/ClO −, have been demonstrated to play essential roles in both physiological and pathological processes, and an abnormal level of HOCl/ClO − is related to some diseases. In this work, a very fast responsive (within 30 seconds) porphyrin-based fluorescent probe, TPP-TCF, for ClO − with a NIR emissive wavelength was prepared. This probe exhibited excellent selectivity towards ClO − and would not be interfered with by other ROS and typical nucleophiles. The limit of detection (LOD) for ClO − was evaluated to be 0.29 μM, indicating high sensitivity towards ClO − . In further bioimaging experiments, TPP-TCF displayed low-cytotoxicity and good cell penetrability for recognizing exogenous ClO − in HeLa cells. Moreover, this probe was successfully applied in imaging endogenous ClO − in living animals.