Graphene-magnetite as adsorbent for magnetic solid phase extraction of 4-hydroxybenzoic acid and 3, 4-dihydroxybenzoic acid in stingless bee honey. (1st November 2018)

• Record Type: Journal Article
• Authors: Musa, Marina; Wan Ibrahim, Wan Aini; Mohd Marsin, Faridah; Abdul Keyon, Aemi S.; Rashidi Nodeh, Hamid
• Is Part Of: Food chemistry. Volume 265(2018)
• Page Start: 165
• Page End: 172
• Publication Date: 2018-11-01
• Subjects: 4-HB 4-Hydroxybenzoic acid -- 3, 4-DHB 3, 4-Dihydroxybenzoic acid -- NH4OH Ammonia solution -- BET Brunauer, Emmet and Teller -- FTIR Fourier transform infrared spectroscopy -- G-Fe3O4 Graphene-magnetite -- HPLC-UV/Vis High performance liquid chromatography with ultravioletvisible detection -- FeCl36H2O Iron(II) chloride tetrahydrate (FeCl24H2O) and iron(III) chloride hexahydrate -- LVSEM Low vacuum scanning electron microscope -- MSPE Magnetic solid phase extraction -- MNPs Magnetic nanoparticles -- Fe3O4 Magnetite -- NaCl Sodium chloride -- TGA Thermogravimetric analysis -- TEM Transmission electron microscope -- VSM Vibrating sample magnetometry -- XRD X-ray diffraction spectroscopy; Stingless bee honey -- Phenolic acids -- Graphene-magnetite -- Magnetic solid phase extraction; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.04.020
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Synthesis of new graphene-magnetite solid phase extraction sorbent (G-Fe3 O4 -MSPE). Fast and green extraction method for two phenolic acids in stingless bee honey. Efficient extraction of phenolic acids with low detection limits (0.08–0.14 µg/g). A method with good relative recoveries (72.6–110.6%) and precision values. Abstract: Graphene-magnetite composite (G-Fe3 O4 ) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3, 4-dihydroxybenzoic acid (3, 4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet–visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3 O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3, 4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6–110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe3 O4 MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples.