Glutathione-driven Cu(i)–O2 chemistry: a new light-up fluorescent assay for intracellular glutathione. Issue 11 (11th May 2018)

• Record Type: Journal Article
• Authors: Gao, Peng Fei; Mao, Yu Ting; Yang, Tong; Zou, Hong Yan; Li, Yuan Fang; Huang, Cheng Zhi
• Is Part Of: Analyst. Volume 143:Issue 11(2018)
• Page Start: 2486
• Page End: 2490
• Publication Date: 2018-05-11
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an00704g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A new intracellular light-up fluorescent assay for glutathione is established, which is based on Cu(i )–O2 chemistry without external H2 O2 . Abstract : Besides its widely known role as an endogenous antioxidant in scavenging free radicals, glutathione (GSH) can also play the role of prooxidant and promote CuO-induced formation of hydroxyl radicals to light up a fluorescent signal through Cu(i )–O2 chemistry without requiring additional H2 O2 . This approach is independent of the mechanisms of enzyme mimics, such as the well-known oxidase and peroxidase mimetics, providing a new method to simply and effectively analyze intracellular GSH.