Glutathione-driven Cu(i)–O2 chemistry: a new light-up fluorescent assay for intracellular glutathione. Issue 11 (11th May 2018) - Gao, Peng Fei
Abstract : A new intracellular light-up fluorescent assay for glutathione is established, which is based on Cu(i )–O2 chemistry without external H2 O2 . Abstract : Besides its widely known role as an endogenous antioxidant in scavenging free radicals, glutathione (GSH) can also play the role of prooxidant and promote CuO-induced formation of hydroxyl radicals to light up a fluorescent signal through Cu(i )–O2 chemistry without requiring additional H2 O2 . This approach is independent of the mechanisms of enzyme mimics, such as the well-known oxidase and peroxidase mimetics, providing a new method to simply and effectively analyze intracellular GSH.