Effect of polyphenols from wild Chilean currants (Ribes spp.) on the activity of intracellular antioxidant enzymes in human gastric AGS cells. (August 2018)

• Record Type: Journal Article
• Authors: Theoduloz, Cristina; Burgos-Edwards, Alberto; Schmeda-Hirschmann, Guillermo; Jiménez-Aspee, Felipe
• Is Part Of: Food bioscience. Volume 24(2018)
• Page Start: 80
• Page End: 88
• Publication Date: 2018-08
• Subjects: 3-CQA 3-caffeoylquinic acid -- AF anthocyanin fraction -- BSA bovine serum albumin -- CAT Catalase -- CF copigment fraction -- DPPH 2, 2-diphenyl-1-picrylhydrazyl radical -- GSH glutathione -- GSH-Px glutathione peroxidase -- GSH-R glutathione reductase -- ORAC oxygen radical absorption capacity -- PEE phenolic-enriched extract -- ROS reactive oxygen species -- SOD superoxide dismutase; Chilean Ribes -- Superoxide dismutase -- Catalase -- Glutathione peroxidase -- Glutathione reductase -- Intracellular total antioxidant activity; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2018.06.003
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: The protective effect of polyphenols from Chilean currants ( Ribes spp.) in human epithelial gastric cells (AGS) against oxidative induced-stress has been recently reported. The aim of the present study was to assess the ability of polyphenol-enriched extracts (PEE) from three Chilean Ribes species to modify the activities of the intracellular antioxidant enzymes: catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R) and superoxide dismutase (SOD) in AGS cells. The PEE of each species was fractionated using membrane chromatography to give an anthocyanin-enriched fraction (AF) and a copigment fraction (CF). A significant increase in the SOD activity was observed with a dose-response effect after an overnight incubation of AGS cells with the PEE, AF and CF from the selected species. The activity of CAT was enhanced by the CF of Ribes magellanicum and R. cucullatum, and by the AF of R. punctatum . For the GSH-R, a dose-response effect was observed in the extracts of R. magellanicum, while for the GSH-Px activity, only the AF of R. magellanicum and R. cucullatum had a significant increase. The studied samples were not able to increase the intracellular GSH content. Only the R. magellanicum samples raised the intracellular total antioxidant activity. These results suggested that the increase in the activity of the antioxidant response enzymes is associated with the cytoprotective effect of these native currants. Graphical abstract: Highlights: The Chilean Ribes extracts and fractions showed strong radical scavenging activities in vitro . Polyphenols from Chilean Ribes species increase the superoxide dismutase activity in AGS cells. Ribes magellanicum extracts and fractions increased the antioxidant activity in AGS cells. Cyanidin-3-glucoside was associated with the increased antioxidant enzyme activities.