Effects of different NaCl concentrations on self-assembly of silver carp myosin. (August 2018)

• Record Type: Journal Article
• Authors: Wang, Guan; Liu, Manman; Cao, Liwei; Yongsawatdigul, Jirawat; Xiong, Shanbai; Liu, Ru
• Is Part Of: Food bioscience. Volume 24(2018)
• Page Start: 1
• Page End: 8
• Publication Date: 2018-08
• Subjects: Myosin -- Self-assembly -- NaCl -- Silver carp (Hypophthalmichthys molitrix); Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2018.05.002
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: The effect of NaCl (0.1 − 3.0 M) on self-assembly of silver carp myosin at 4 °C was investigated in terms of microstructure, conformation, intermolecular interactions, and particle size distribution. During setting at 4 °C, the self-assembly of myosin showed an obvious concentration dependence. At low concentrations (<0.3 M), myosin assembled into filaments mainly through rod-rod ionic linkages, and showed significantly (P < 0.05) higher turbidity and lower solubility than at ≥0.3 M. The addition of NaCl (0.3 − 0.6 M) led to the extension and dissolution of myosin by interfering with electrostatic interactions. The myosin assemblies gradually became smaller and more uniform. The solubility and Ca 2+ -ATPase activity reached a maximum at 0.6 M NaCl. When the concentrations increased to >1.0 M, myosin further assembled into filaments dominated by hydrophobic interactions. Both rod-rod and head-head interactions contributed to the myosin filaments at high concentrations (1.0 − 3.0 M). The solubility and uniformity of the myosin assemblies decreased as the NaCl concentration increased from 1.0 to 3.0 M. Highlights: Myosin self-assembled to filaments mainly through rod-rod ionic linkages at low NaCl concentrations (<0.3 M). Myosin assembled into filaments dominated by hydrophobic interactions at high NaCl concentrations (1.0–3.0 M). The assemblies at 1.0–3.0 M NaCl were more uniform and more compact than those at 0.1 M NaCl.