Biochemical evaluation of selected grape varieties (Vitis vinifera L.) grown in Jordan and in vitro evaluation of grape seed extract on human prostate cancer cells. (August 2018)

• Record Type: Journal Article
• Authors: El-Elimat, Tamam; Jarwan, Bara`a A.; Zayed, Aref; Alhusban, Ahmed; Syouf, Maha
• Is Part Of: Food bioscience. Volume 24(2018)
• Page Start: 103
• Page End: 110
• Publication Date: 2018-08
• Subjects: Grapes -- Resveratrol -- Vitis vinifera -- Vitaceae -- Golden Scatt -- Human prostate cancer cells; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2018.06.007
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: A chemical and biological approach was used for quality assessment of 16 different eating grape varieties collected in Jordan. A validated LC-ESI-MS/MS method was used for quantitative analysis of resveratrol in grape skin and grape seed extracts. Grape skin extracts showed higher resveratrol content than that of seeds. The antioxidant activity and total phenolic content of seed, skin, and whole berry extracts of the grape varieties were evaluated using the DPPH radical scavenging assay and Folin–Ciocalteu colorimetric method, respectively. Seed extracts showed the highest total phenolic content and antioxidant activity. The in vitro effects of Golden Scatt grape seed extract on human prostate cancer cell migration and colony-genic potential were assayed. Golden Scatt seed extract modulated the colony formation potential and inhibited the migratory potential of prostate cancer cells in a dose-dependent manner. Graphical abstract: Highlights: A chemical and biological approach was applied for quality assessment of grapes. A validated LC-ESI-MS/MS method was used for quantitative analysis of resveratrol. The antioxidant activity and total phenolic content of grapes were evaluated. In vitro effects of grape seed on human prostate cancer (PC3) cell migration. In vitro effects of grape seed on the colony-genic potential of PC3 cells.