Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk. (September 2018)

• Record Type: Journal Article
• Authors: Du, Meihong; Li, Jingwen; Zhao, Ruixue; Yang, Yin; Wang, Yanfei; Ma, Kai; Cheng, Xiaoyan; Wan, Yuping; Wu, Xiaosheng
• Is Part Of: Food control. Volume 91(2018)
• Page Start: 92
• Page End: 99
• Publication Date: 2018-09
• Subjects: Immune-magnetic beads (IMBs) -- Immune-magnetic separation (IMS) -- Pre-treatment -- Salmonella -- Rapid detection; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2018.03.032
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella . Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 10 6 CFU/mL of Salmonella . IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10 −1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. Highlights: A pre-treatment method based on immune-magnetic separation with culture is proposed. Immune-magnetic separation is prior to culture in pretreatment procedure. The 25 mL large system is adopted for Immune-magnetic separation. Pretreatment method could be integrated with many rapid detection techniques. Rapid detection of trace levels of Salmonella has been achieved.