Doubled haploid wheat lines with high molecular weight glutenin alleles derived from microspore cultures. Issue 3 (3rd July 2018)

• Record Type: Journal Article
• Authors: Barakat, Mohamed N.; Al-Doss, Abdullah A.; Ghazy, Abdelhalim I.; Moustafa, Khaled A.; Elshafei, Adel A.; Ahmed, Eid I.
• Is Part Of: New Zealand journal of crop and horticultural science. Volume 46:Issue 3(2018)
• Page Start: 198
• Page End: 211
• Publication Date: 2018-07-03
• Subjects: Bread quality -- doubled haploid -- microspore cultures -- high molecular wight glutenin subunits (HMW-GS) -- wheat; Crops -- New Zealand -- Periodicals; Horticulture -- New Zealand -- Periodicals; Crops -- Periodicals; Horticulture -- Periodicals; Crops; Horticulture; New Zealand; Periodicals; 633.09905
• DOI: 10.1080/01140671.2017.1368674
• Languages: English
• ISSNs: 1175-8783

Abstract

ABSTRACT: A doubled haploid (DH) production system for wheat using a microspore culture method produced seeds and subsequently regenerated haploid wheat plants derived from the F1 plants of two wheat populations (Irena × Ksu102 and Klasic × Ksu105). 68 DH were obtained from both populations. Field trials of these DH lines were conducted over two seasons. Genotypic differences were highly significant for all agronomic traits recorded. Sets of five gene- or allele-specific PCR markers were used for identification of genes encoding high molecular weight (HMW) glutenin subunits Ax1 and Ax2* at the Glu-A1 locus, Bx7, Bx7*, By8 and By17 at the Glu-B1 locus and Dx5, Dy10 and Dy12 at the Glu-D1 locus in wheat inbred lines. The method developed for providing DH lines and subsequent molecular analysis and quality could be useful for improving bread-making quality and developing new cultivars. Abbreviations: DH: doubled haploid; HMW: high molecular weight; HMW-GS: high molecular weight glutenins subunits; MAB: marker-assisted breeding