Doubled haploid wheat lines with high molecular weight glutenin alleles derived from microspore cultures. Issue 3 (3rd July 2018) - Barakat, Mohamed N.
ABSTRACT: A doubled haploid (DH) production system for wheat using a microspore culture method produced seeds and subsequently regenerated haploid wheat plants derived from the F1 plants of two wheat populations (Irena × Ksu102 and Klasic × Ksu105). 68 DH were obtained from both populations. Field trials of these DH lines were conducted over two seasons. Genotypic differences were highly significant for all agronomic traits recorded. Sets of five gene- or allele-specific PCR markers were used for identification of genes encoding high molecular weight (HMW) glutenin subunits Ax1 and Ax2* at the Glu-A1 locus, Bx7, Bx7*, By8 and By17 at the Glu-B1 locus and Dx5, Dy10 and Dy12 at the Glu-D1 locus in wheat inbred lines. The method developed for providing DH lines and subsequent molecular analysis and quality could be useful for improving bread-making quality and developing new cultivars. Abbreviations: DH: doubled haploid; HMW: high molecular weight; HMW-GS: high molecular weight glutenins subunits; MAB: marker-assisted breeding