Characterisation of polyphenol constituents of Linderae aggregate leaves using HPLC fingerprint analysis and their antioxidant activities. (1st November 2015)

• Record Type: Journal Article
• Authors: Xu, Chanjuan; Yang, Bingxian; Zhu, Wei; Li, Ximin; Tian, Jingkui; Zhang, Lin
• Is Part Of: Food chemistry. Volume 186(2015)
• Page Start: 83
• Page End: 89
• Publication Date: 2015-11-01
• Subjects: Linderae aggregate -- Polyphenol -- Flavonoid -- Quality control -- Antioxidant assays; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.11.042
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Polyphenol profile of LAL was clarified using HPLC–DAD–MS analysis for the first time. HPLC fingerprint data of 11 common peaks in LAL were established. Contents and antioxidant properties of flavonoids were determined. Optimal collection time and quality control method of LAL were recommended. Abstract: The leaves of Linderae aggregate (LAL) has been used as a type of tea in China and other Southeast Asian countries. In this study, 11 polyphenols in LAL were clarified for the first time using multiple high-performance liquid chromatographic techniques. An optimal extracting method was developed through the comparison of the amount of quercetin-3- O -α-l -rhamnoside using a uniform design method. From the fingerprint liquid chromatography data, 11 common peaks in the 8 samples collected from April to November were semi-determined. The antioxidant capacities were examined using the 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay and the ferric reducing/antioxidant power assay. All 8 samples contained the same 11 polyphenols in similar ratios. Three samples, S2, S5 and S6 contained higher amount of quercetin-3- O -α-l -rhamnoside and were demonstrated to have stronger antioxidant capacities in both antioxidant assays. These results are critical in optimising harvest time and quality control of LAL.