Chemical composition, total phenolic content, antioxidant and antinutritional characterisation of exudate gums. (June 2018)

• Record Type: Journal Article
• Authors: Hamdani, Afshan Mumtaz; Wani, Idrees Ahmed; Bhat, Naseer Ahmad; Masoodi, F.A.
• Is Part Of: Food bioscience. Volume 23(2018)
• Page Start: 67
• Page End: 74
• Publication Date: 2018-06
• Subjects: Apricot -- Exudate -- Antioxidant -- Antinutritional -- Tannin; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2018.03.006
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Present study was conducted to determine the chemical composition, antioxidant potential and antinutritional factors in some plant gums that have been commercially used as additives in different food formulations. For this purpose, exudate gums were chosen which included commercially used acacia and karaya gum. The properties were compared with an underutilised gum exuded from apricot tree, called apricot gum. Monosaccharide composition and linkages in gum polysaccharides were studied using Electron spray ionization-mass spectrometry coupled with time-of-flight analyser (ESI-MS-TOF). The assay involves ionization of gum polysaccharides by passing their organic solution into electric field and converting them into a mist of charged droplets that are further analysed and detected using Time-of-flight mass spectrometry. Relative array of peaks elucidated the presence of glucosyl, galactosyl and a small proportion of uronic acid units in all gums. Spectral fragmentation distribution was bimodal with polydispersity values greater than or equal to two (Mw /Mn ≥ 2). In all the antioxidant assays, methanol and ethanol were separately used for extraction. Total phenolic content, ferrous ion chelating ability and reducing power of the gums varied from 0.44 to 1.25 mg GAE/g, 11.80–60.42% and 0.12–0.23 AAE/g, respectively. The DPPH radical scavenging ability (12.03–27.23%) and bile acid binding activity (40.98–46.60%) of the gums was also high. Presence of tannins (0.20–0.73 TAE/g), phytic acid (0.03–0.60 mg/g) and haemagglutinins (0.006–0.11 HU/mg protein) was observed at a very small concentration. On the other hand, the antinutritional factors like terpenoids, saponins and alkaloids were found completely absent. Graphical abstract: Highlights: Gums had high antioxidant potential. Ferrous ion chelating ability of gums ranged from 11.80% to 60.42%. % inhibition of DPPH radical was also high (12.03–27.23). Apricot gum exhibited the highest antioxidant potential. Terpenoids, saponins and alkaloids were absent in gums.