Considering the potential of Lactobacillus rhamnosus for producing Angiotensin I-Converting Enzyme (ACE) inhibitory peptides in fermented camel milk (Indian breed). (June 2018)

• Record Type: Journal Article
• Authors: Solanki, Divyang; Hati, Subrota
• Is Part Of: Food bioscience. Volume 23(2018)
• Page Start: 16
• Page End: 22
• Publication Date: 2018-06
• Subjects: Camelus dromedarius -- ACE-inhibitory activity -- PepX activity -- Camel milk; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2018.03.004
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: In this study, fermented camel milk was prepared using proteolytic Lactobacillus rhamnosus MTCC 5945 (NS4), which was analyzed for X-prolyl-dipeptidyl aminopeptidase activity and Angiotensin I-Converting Enzyme (ACE) inhibitory activity. The growth conditions (inoculation rate and incubation time) for the production of peptides were optimized using OPA method. Fractionated 3 kDa, 5 kDa and 10 kDa permeate and retentate samples were further analyzed for ACE-inhibitory activity under the optimized growth conditions. Furthermore, 3 kDa and 10 kDa permeate with highest ACE-inhibitory activities and highest percentage of peptides production were subjected to liquid chromatography mass spectrometry and peptide identification using MASCOT software. Novel peptides were identified from fermented camel milk using homology sequence searching in BlastP (NCBI), and Protein information resource database (PIR). The novelty (ACE-inhibitory activity or Antihypertensive activity) of peptides was also confirmed using novelty search in the database of antihypertensive peptides (AHTPDB). Highlights: Traditionally, camel milk is served as a biomedicine since long. Fermented camel milks contain physiologically bioactive components. Fermented Camel milk could be act as probiotics products with multi functionality. ACE-inhibitory smaller peptides were identified from fermented camel milk.