Highly sensitive furazolidone monitoring in milk by a signal amplified lateral flow assay based on magnetite nanoparticles labeled dual-probe. (30th September 2018)

• Record Type: Journal Article
• Authors: Yan, Lingzhi; Dou, Leina; Bu, Tong; Huang, Qiong; Wang, Rong; Yang, Qingfeng; Huang, Lunjie; Wang, Jianlong; Zhang, Daohong
• Is Part Of: Food chemistry. Volume 261(2018)
• Page Start: 131
• Page End: 138
• Publication Date: 2018-09-30
• Subjects: Lateral flow assay -- Magnetite nanoparticles -- Signal amplification -- Furazolidone; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.04.016
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: We presented a signal amplified lateral flow assay based on MNPs labeled dual-probe for furazolidone residue monitoring. The signal amplified strip test providing a 10-fold improvement of sensitivity than the traditional AuNP based strip tests. The proposed signal enhancement strategy was easier than previous reported methods. Abstract: We presented a signal amplified lateral flow assay (LFA) based on magnetite nanoparticles (MNPs) labeled dual-probe and applied it in the high sensitive and rapid on-site detection of furazolidone metabolite of 3-amino-2-oxazolidinone (AOZ). The amplified signal benefited from high affinity between two probes of MNPs labeled murine monoclonal antibody (MNPs-MAb) and goat anti-mouse antibody (MNPs-GAMA) and was achieved by the generation of dual-probe network complex. This developed method could realize high sensitive detection of AOZ with a threshold value of 0.88 ng mL −1 and a detection limit of 0.044 ng mL −1, the sensitivity was at least 10-fold improved than that of the traditional gold nanoparticle based LFA. This facile developed assay was successfully applied for rapid detection of AOZ in milk samples. The proposed method paves a new way for on-site screening of other hazardous substances in food and can be referred in all lateral flow assays.