Essential oils (EOs), pressurized liquid extracts (PLE) and carbon dioxide supercritical fluid extracts (SFE-CO2) from Algerian Thymus munbyanus as valuable sources of antioxidants to be used on an industrial level. (15th September 2018)

• Record Type: Journal Article
• Authors: Bendif, Hamdi; Adouni, Khaoula; Miara, Mohamed Djamel; Baranauskienė, Renata; Kraujalis, Paulius; Venskutonis, Petras Rimantas; Nabavi, Seyed Mohammad; Maggi, Filippo
• Is Part Of: Food chemistry. Volume 260(2018)
• Page Start: 289
• Page End: 298
• Publication Date: 2018-09-15
• Subjects: TMC Thymus munbyanus subsp. Coloratus -- TMM Thymus munbyanus subsp. Munbyanus -- AAPH 2, 2-azobis-(2-amidino-propane) dihydrochloride -- AA antioxidant activity -- DPPH 2, 2-diphenyl-1-picrylhydrazyl radical -- DWP dry weight of plant material -- DWE Dry weight of extract -- GAE Gallic acid equivalents -- H-ORAC Hydrophilic-oxygen radical absorbance capacity -- l-ORAC Lipophilic-oxygen radical absorbance capacity -- EOs essential oils -- PLE Pressurized liquid extraction -- ASE accelerated solvent extraction -- SFE-CO2 Supercritical fluid extraction -- TE Trolox equivalents -- TEAC Trolox equivalent antioxidant capacity -- TPC Total phenolic content -- GC-FID Gas chromatography-Flame ionization detector -- FRAP Ferric reducing antioxidant power -- HPLC high performance liquid chromatography -- GC×GC-TOFMS Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry -- AC acetone -- ET ethanol -- W water; Thymus munbyanus subsp. coloratus -- Thymus munbyanus subsp. munbyanus -- EOs -- SFE-CO2 -- PLE -- Antioxidant; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.03.108
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Thymus munbyanus subsp. coloratus and munbyanus were studied. Essential oils, pressurized liquid and supercritical fluid extracts were obtained. Extracts were examined for total phenolic and antioxidant activities. Pressurized liquid extracts had the strongest antioxidant capacity. Abstract: The aim of this study was to demonstrate the potential of extracts from Algerian Thymus munbyanus as a valuable source of antioxidants for use on an industrial level. To this end, a study was conducted on the composition and antioxidant activities of essential oils (EOs), pressurized liquid extracts (PLE) and supercritical fluid extracts (SFE-CO2 ) obtained from Thymus munbyanus subsp . coloratus (TMC) and subsp . munbyanus (TMM). EOs and SFE-CO2 extracts were analysed by GC-FID and GC×GC-TOFMS revealing significant differences. A successive extraction of the solid SFE-CO2 residue by PLE extraction with solvents of increasing polarity such as acetone, ethanol and water, was carried out. The extracts were evaluated for total phenolic content by Folin–Ciocalteu assay, while the antioxidant power was assessed by DPPH, FRAP, and ORAC assays. SFE-CO2 extracts were also analysed for their tocopherol content. The antioxidant activity of PLE extracts was found to be higher than that of SFE-CO2 extracts, and this increased with solvent polarity (water > ethanol > acetone). Overall, these results support the use of T. munbyanus as a valuable source of substances to be used on an industrial level as preservative agents.