A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint. (1st October 2015)

• Record Type: Journal Article
• Authors: Guo, ZhenZhong; Zine, Nadia; Lagarde, Florence; Daligault, Julien; Persuy, Marie-Annick; Pajot-Augy, Edith; Zhang, Aidong; Jaffrezic-Renault, Nicole
• Is Part Of: Food chemistry. Volume 184(2015)
• Page Start: 1
• Page End: 6
• Publication Date: 2015-10-01
• Subjects: Olfactory receptor -- Functional immobilization -- Fluorescence microscopy -- Square wave voltammetry; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.03.066
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A strategy for the immobilization of ORs on a gold surface is developed. Olfactory receptor/odorant recognition process is monitored using SWV. Dose-dependent detection of androstenone in solution is obtained. Unrelated odorant, helional is not detected. Abstract: We report a dose-dependent detection of androstenone in solution, as one of the boar taint compounds, based on related OR7D4 olfactory receptors immobilized on a gold electrode through their 6-His tag and NTA–copper complex, as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is for the first time, the method used to monitor the olfactory receptor/odorant recognition process. The relative variation of the Cu(I)–Cu(II) current peak increases linearly versus log (concentration of androstenone) from 10 −14 M to 10 −4 M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.