Identification of sea cucumber species in processed food products by PCR-RFLP method. (August 2018)

• Record Type: Journal Article
• Authors: Zeng, Ling; Wen, Jing; Fan, Sigang; Chen, Ziming; Xu, Youhou; Sun, Yulin; Chen, Daohai; Zhao, Juan; Xu, Lele; Li, Yongqin
• Is Part Of: Food control. Volume 90(2018)
• Page Start: 166
• Page End: 171
• Publication Date: 2018-08
• Subjects: Sea cucumbers -- Species identification -- 16S rRNA gene -- PCR-RFLP -- FINS; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2018.02.048
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: A PCR-RFLP method has been developed for the identification of 16 commercial sea cucumber species in raw and processed food products. To implement the method, PCR amplification with the 16Sar/16Sbr primers, targeted to the amplification of a ca. 570 bp region of the 16S rRNA mitochondrial gene in sea cucumbers, was coupled to restriction analysis with Dde I, Hae III and Sty I. We also report the FINS method based on 16S rRNA mitochondrial sequences. Both proposed methodologies were independently applied to authenticate the species of 19 commercial products, showing that 9 products were incorrectly labeled (48%). Therefore, the 16S rRNA mitochondrial gene really provide convenient, useful and academic molecular marker to study questions related to correct labeling of commercial products, traceability, and the control of sea cucumber fisheries. Highlights: The method allows the identification of commercial sea cucumber species in fresh, frozen and dried forms. 48% of the samples were incorrectly labeled. This method showed good sensitivity and accuracy.