Prevalence, sequence types, antibiotic resistance and, gyrA mutations of Salmonella isolated from retail fresh chicken meat in Singapore. (August 2018)

• Record Type: Journal Article
• Authors: Zwe, Ye Htut; Tang, Vivien Chia Yen; Aung, Kyaw Thu; Gutiérrez, Ramona Alikiiteaga; Ng, Lee Ching; Yuk, Hyun-Gyun
• Is Part Of: Food control. Volume 90(2018)
• Page Start: 233
• Page End: 240
• Publication Date: 2018-08
• Subjects: Salmonella -- Chicken -- Sequence types -- Antibiotic resistance -- Quinolone resistance; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2018.03.004
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Salmonellosis, caused by multidrug-resistant Salmonella species, in particular, is one of the leading notifiable foodborne diseases in Singapore and an emerging public health concern worldwide. The objectives of this study were to determine the prevalence, antibiotic resistance and the presence of gyrA and parC mutations in Salmonella isolated from retail fresh chicken meat in Singapore. A significantly higher prevalence of Salmonella was found in chicken meat from the wet markets (25%, 30/120), as compared to supermarkets (12.7%, 19/150). The top four serovars isolated in this study were S. Saintpaul (32.7%, 17/52), followed by S. Brancaster (21.2%, 11/52), S. Albany (11.5%, 6/52), and S. Stanley (9.6%, 5/52). More than 80% of Salmonella isolates exhibited resistance to at least one of eleven antibiotics tested. The most common phenotypic resistances exhibited were towards ampicillin (78.8%, 41/52), tetracycline and chloramphenicol (61.5%, 32/52), sulfamethoxazole-trimethoprim (55.8%, 29/52) and nalidixic acid (30.8%, 16/52). Of the 52 Salmonella isolates, 59.6% (31/52) were multi-drug resistant strains, resistant to 3 or more antibiotic classes. No mutation in parC gene was found in any of the isolates that expressed phenotypic resistance or reduced susceptibility towards quinolone and/or fluoroquinolone. Mutations at two different sites of gyrA gene were found in 7 isolates, which further discriminates the quinolone resistance genotype in Salmonella isolates from fresh chicken meat. Our findings provide opportunities for risk assessment and management of salmonellosis and antibiotic-resistant Salmonella species in the country and the region. Highlights: Salmonella contamination was higher in the wet markets than supermarkets. S. Saintpaul (32.7%) was most prevalent, followed by S. Brancaster (21.2%). Multi-drug resistance rate of Salmonella was 60%. Two separate mutations in gyrA for quinolone resistance were found.