Urinary Excretion of Niacin Metabolites in Humans After Coffee Consumption. Issue 7 (23rd March 2018)

• Record Type: Journal Article
• Authors: Kremer, Jonathan Isaak; Gömpel, Katharina; Bakuradze, Tamara; Eisenbrand, Gerhard; Richling, Elke
• Is Part Of: Molecular nutrition & food research. Volume 62:Issue 7(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-03-23
• Subjects: coffee -- metabolism -- niacin -- nicotinamide -- stable isotope dilution analysis (SIVA); Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201700735
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Coffee is a major natural source of niacin in the human diet, as it is formed during coffee roasting from the alkaloid trigonelline. The intention of our study was to monitor the urinary excretion of niacin metabolites after coffee consumption under controlled diet. Methods and results: We performed a 4‐day human intervention study on the excretion of major niacin metabolites in the urine of volunteers after ingestion of 500 mL regular coffee containing 34.8 μmol nicotinic acid (NA) and 0.58 μmol nicotinamide (NAM). In addition to NA and NAM, the metabolites N 1 ‐methylnicotinamide (NMNAM), N 1 ‐methyl‐2‐pyridone‐5‐carboxamide (2‐Py), and nicotinuric acid (NUA) were identified and quantified in the collected urine samples by stable isotope dilution analysis (SIVA) using HPLC‐ESI‐MS/MS. Rapid urinary excretion was observed for the main metabolites (NA, NAM, NMNAM, and 2‐Py), with t max values within the first hour after ingestion. NUA appeared in traces even more rapidly. In sum, 972 nmol h −1 of NA, NAM, NMNAM, and 2‐Py were excreted within 12 h after coffee consumption, corresponding to 6% of the ingested NA and NAM. Conclusion: The results indicate regular coffee consumption to be a source of niacin in human diet. Abstract : Coffee is a major source of niacin (nicotinic acid and nicotinamide) in the human diet, as it is formed during coffee roasting from the alkaloid trigonelline. This study focused on the renal excretion of nicotinic acid and nicotinamide, together with the niacin metabolites nicotinuric acid, N 1 ‐methylnicotinamide, and N 1 ‐methyl‐2‐pyridone‐5‐carboxamide in human volunteers after consumption of 500 mL freshly brewed coffee.