HPLC analysis of six iridoid glycosides from Cymbaria dahurica L. and their structural elucidation. Issue 2 (30th November 2017)

• Record Type: Journal Article
• Authors: Wang, Qinghu; Bao, Yinping; Hao, Junsheng; Han, Jingjing
• Is Part Of: Journal of food biochemistry. Volume 42:Issue 2(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-11-30
• Subjects: Cymbaria dahurica L -- cymdahoside A -- HPLC -- iridoid glycoside; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.12470
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: Studies on ethanol extract from Cymbaria dahurica L. ( C. dahurica ) resulted in isolation and identification of a new iridoid glycoside together with five known compounds. Moreover, a high‐performance liquid chromatographic method has been developed for the simultaneous determination of six iridoid glycosides in C. dahurica . The separation by gradient elution was performed on Inertsil ODS‐SP column (250 mm × 4.6 mm, 5 µm) at 30°C with acetonitrile and water as the mobile phase, and monitored by absorbance at 210 nm. The recovery of the method is from 95.0 to 98.0%, and linearity ( r > .9994) was obtained for six compounds. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.6%) were also achieved. The validated method was successfully applied to the determination of six compounds in six samples. Practical applications: The aerial parts of Cymbaria dahurica has been used as foods and herbal remedies in Inner Mongolia of China, which has a wide range of health benefits, significantly antioxidant properties. In previous phytochemical studies, non‐glycosidic iridoids and flavonoids were isolated. Iridoids are a class of secondary metabolites with the significant pharmacological activities including antitumor, antioxidant, and anti‐inflammatory. Therefore, a detailed analysis and recognition of structural compounds diversity in C. dahurica is of great importance to evaluate their biological activities.