A monoclonal antibody based enzyme-linked immunosorbent assay for detection of phenylethanolamine A in tissue of swine. (15th January 2015)

• Record Type: Journal Article
• Authors: Li, Yansong; Lu, Shiying; Liu, Zengshan; Sun, Lihui; Guo, Junfei; Hu, Pan; Zhang, Junhui; Zhang, Yuanyuan; Wang, Yang; Ren, Honglin; Meng, Xingyu; Zhou, Yu
• Is Part Of: Food chemistry. Volume 167(2015)
• Page Start: 40
• Page End: 44
• Publication Date: 2015-01-15
• Subjects: Phenylethanolamine A (PEA) -- Monoclonal antibody (McAb) -- Assay; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.06.085
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A McAb specific for PEA was produced. An ELISA based on the McAb for PEA detection tissue of swine was developed. The average recovery of PEA from spiked samples was 103.4%. Abstract: Phenylethanolamine A (PEA) is a phenethanolamine member of the family of β-adrenergic agonists compounds illegally used as feed additives for growth promotion. In this study, PEA was covalently linked to carrier protein cationized bovine serum albumin (cBSA) and egg albumin (OVA). A monoclonal antibody specific for PEA was produced and characteristics of monoclonal antibody (McAb) were studied. Based on the McAb, an enzyme-linked immunosorbent assay (ELISA) for detection of PEA was developed, which showed an IC50 value of 6.25 ng mL −1 and a detection limit of 0.19 ng mL −1 . The average recovery of PEA from spiked samples was 103.4%, which demonstrated that the matrices of meat where PEA may be found do not interfere with the assay.