Antidiabetic Activity of Ergosterol from Pleurotus Ostreatus in KK‐Ay Mice with Spontaneous Type 2 Diabetes Mellitus. Issue 3 (9th January 2018)

• Record Type: Journal Article
• Authors: Xiong, Mingrui; Huang, Yun; Liu, Yajing; Huang, Mi; Song, Guanjun; Ming, Qian; Ma, Xinhua; Yang, Jie; Deng, Shihao; Wen, Yanzhang; Shen, Jinhua; Liu, Qing‐Hua; Zhao, Ping; Yang, Xinzhou
• Is Part Of: Molecular nutrition & food research. Volume 62:Issue 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-01-09
• Subjects: diabetic mice -- ergosterol -- GLUT4 -- hypoglycemic agents -- p‐Akt -- p‐PKC; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201700444
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: The number of people with diabetes is increasing rapidly in the world. In the present study, the hypoglycemic activity and potential mechanism of ergosterol (ERG), a phytosterol derived from the edible mushroom Pleurotus ostreatus are investigated in vitro and in vivo. Methods and results: ERG is isolated from Pleurotus ostreatus and identified by NMR spectra. The effects of ERG on the glucose uptake, glucose transporter 4 (GLUT4) translocation, GLUT4 expression, and the phosphorylation of AMPK, Akt and PKC in L6 cells are evaluated. ERG enhances glucose uptake and displays a GLUT4 translocation activity with up‐regulating GLUT4 expression and phosphorylation of Akt and PKC in L6 cells. In vivo, antidiabetic activity of ERG is examined. The phosphorylation of Akt and PKC in different tissues from KK‐A y mice is assessed. ERG significantly improves insulin resistance and blood lipid indices while reducing fasting blood glucose levels and protecting pancreas and liver in the mice. Moreover, the phosphorylation of Akt and PKC is increased in different tissues. Conclusion: The results suggest that ERG may be a potential hypoglycemic agent for the treatment of T2DM with the probable mechanism of stimulating GLUT4 translocation and expression modulated by the PI3K/Akt pathway and PKC pathway. Abstract : Glucose transporter 4 (GLUT4) is an important protein for glucose homeostasis, which is a target in the treatment of type 2 diabetes (T2DM). This research shows that Ergosterol (ERG) has an antidiabetic effect in KK‐A y mice and ERG can promote GLUT4 translocation, GLUT4 expression, and glucose uptake via the PI3K/Akt and PKC pathways in L6 cells and mice.