Chemical characterization and anti-hyperglycaemic effects of polyphenol enriched longan (Dimocarpus longan Lour.) pericarp extracts. (March 2015)

• Record Type: Journal Article
• Authors: Li, Liya; Xu, Jialin; Mu, Yu; Han, Li; Liu, Ruohan; Cai, Yaping; Huang, Xueshi
• Is Part Of: Journal of functional foods. Volume 13(2015)
• Page Start: 314
• Page End: 322
• Publication Date: 2015-03
• Subjects: LPE Longan pericarp extracts -- DM diabetes mellitus -- TCM Traditional Chinese Medicine -- LC liquid chromatography -- MS mass spectrometry -- HPLC high performance liquid chromatography -- DAD diode array detector -- PNPG p-nitrophenyl-α-D-glucopyranoside -- MRM multiple reaction monitoring -- GAEs gallic acid equivalents -- DW dry weight -- AUC area under curve -- Irs-1 insulin receptor substrate 1 -- Pparγ peroxisome proliferator-activated receptor γ -- Glut4 glucose transporter 4 -- IGT impaired glucose tolerance; Anti-hyperglycaemic -- α-Glucosidase -- Dimocarpus longan Lour -- Polyphenol -- Chemical composition; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.01.006
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Longan pericarp extracts reduce postprandial hyperglycaemia by inhibiting α-glucosidase in the gut lumen. Longan pericarp extracts enhance mouse glucose tolerance with short-term oral administration. Longan pericarp extracts enhance insulin sensitivity via increasing Irs-1, Pparγ and Glut4 gene expression. Abstract: The chemical composition and anti-hyperglycaemic effects of polyphenol-enriched Longan pericarp extracts (LPE) were investigated in the present study. The phenolic profile of LPE was determined by HPLC-DAD/MS analysis. Nine phenolics were identified, and of these, three major components were quantified by LC-MS/MS. Anti-hyperglycaemic assays demonstrate that LPE (polyphenol content ca. 37.8 g gallic acid equivalents/100 g) inhibit α-glucosidase (IC50 = 11.68 ± 0.44 µg/mL) in vitro and decrease blood glucose content in mice (P < 0.05) upon sucrose challenge. In addition, LPE improves mouse glucose tolerance following 5 days of treatment by oral gavage (10 mg/kg body weight), which may be due to the increased gene expression associated with insulin signalling pathway, such as insulin receptor substrate-1, peroxisome proliferator-activated receptor γ and glucose transporter 4. Overall, our results suggest that LPE could impact carbohydrate metabolism and enhance insulin sensitivity and may serve as a potential anti-hyperglycaemic agent in diabetes care.