Quality control of commercial cranberry products: HPTLC-densitometry a new deal. (April 2018)

• Record Type: Journal Article
• Authors: Boudesocque-Delaye, Leslie; Lanoue, Arnaud; Dorat, Joëlle; Bruyère, Frank; Gueiffier, Alain; Enguehard-Gueiffier, Cécile
• Is Part Of: Food control. Volume 86(2018)
• Page Start: 214
• Page End: 223
• Publication Date: 2018-04
• Subjects: Cranberry -- HPTLC -- UPLC-MS -- Proanthocyanidin -- Quality control; Epicatechin (PubChem CID: 72276) -- Catechin (PubChem CID: 73160) -- Proanthocyanidin A2 (PubChem CID: 124025) -- Proanthocyanidin B2 (PubChem CID: 122738) -- 4-Dimethylaminocinnamaldehyde (PubChem CID: 5284506); Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.11.017
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: A fast quality control protocol based on reference BL-DMAC assay and High-performance thin-layer chromatography (HPTLC)-Densitometry was optimized for routine quality control of cranberry ( Vaccinium macrocarpon ) products. The sample preparation appeared as a key step to ensure a relevant comparison of products quality and biological data. Sequential solid/liquid extractions were required to fully extract polyphenols, regardless the extraction solvent chosen. The HPTLC-densitometry herein protocol used epicatechin, PAC-A2 and PAC-B2 as quality markers and densitometric measurements at 200 nm. The PAC-A2 /Epicatechin ratio, obtained using HPTLC, appeared as an appropriate indicator of the quality of cranberry ingredient. Only two products, among the tested panel, exhibited a high quality cranberry-based ingredient using this criteria. UPLC-MS analyses coupled to multivariate analyses confirmed HPTLC-densitometry conclusions. This work highlighted the plural PAC composition of commercial products and the lack of standardization for cranberry-based products on the market, explaining the non-reproducibility of clinical trials. Highlights: HPTLC-densitometry protocol was optimized for low concentrated PACs products. Solid/liquid extraction appeared as a key step. This work highlighted the plural PAC composition of commercial products. UPLC-MS combined to multivariate analysis confirmed HPTLC data. PAC-A2 /Epicatechin ratio >2 is a pertinent quality indicator.