Fluorescent sensor assay for β-lactamase in milk based on a combination of aptamer and graphene oxide. (March 2017)

• Record Type: Journal Article
• Authors: Qin, Jing; Cui, Xiping; Wu, Panpan; Jiang, Zhengyun; Chen, Yingshan; Yang, Ruili; Hu, Qingqing; Sun, Yuanming; Zhao, Suqing
• Is Part Of: Food control. Volume 73:Part B(2017)
• Page Start: 726
• Page End: 733
• Publication Date: 2017-03
• Subjects: Fluorescence sensor assay -- β-lactamase -- Aptamer -- Graphene oxide; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2016.09.023
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Fluorescent sensor assay (FSA) was developed by using a fluorescein-labeled aptamer assembled onto Graphene oxide (GO) in order to determine β-lactamase in milk. Under optimal conditions, FSA indicated a detection range from 1 to 46 U/mL with a limit of detection (LOD) of 0.5 U/mL ( R 2 = 0.999, n = 3). In addition, commercial milk samples tainted with β-lactamase were detected by the established FSA with a recovery rate between 96.04 and 119.67%. Additionally, the reliability and sensitivity of FSA was validated by enzyme-linked immunoassay (ELISA) with a high correlation of 0.993. Thus, these data, combined with the ease and speed of the assay, suggest that the developed FSA may represent a promising method for monitoring β-lactamase contamination in milk. Highlights: Graphene oxide (GO) is proposed to be able to quench the fluorescence signal. β-lactamase aptamer was used to replace the traditional antibody. Based on aptamer assembled onto GO, a new, sensitive and direct determination of β-lactamase was established. The established method was verified by ELISA.