Comparison of secondary metabolite changes in Camellia sinensis leaves depending on the growth stage. (March 2017)

• Record Type: Journal Article
• Authors: Ryu, Hyung Won; Yuk, Heung Joo; An, Ju Hyeon; Kim, Doo-Young; Song, Hyuk-Hwan; Oh, Sei-Ryang
• Is Part Of: Food control. Volume 73:Part B(2017)
• Page Start: 916
• Page End: 921
• Publication Date: 2017-03
• Subjects: Camellia sinensis -- Secondary metabolite -- Growth stage -- UPLC–QTof MS; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2016.10.017
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: To improve tea leaves' utility as a functional crop, it is necessary to understand the distribution and alteration of bioactive secondary metabolites during different growth stages. In the present study, secondary metabolites changes in C. sinensis leaves were investigated according to growth stage using ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC–QTof MS) with multivariate analysis. Through principal component analysis of the metabolites, patterns could be distinguished from samples harvested between April and August. On the loading plot, significant changes in the contents of metabolites were found during growth, and two gallotannins (4 and10 ), three flavan-3-ols (6, 7, and17 ), two flavonols (14 and16 ), and two theaflavins (29 and31 ) were evaluated as growth markers among thirty-one isolated metabolites. In particular, the flavonols and theaflavins increased gradually, whereas gallotannins and flavan-3-ols decreased continuously depending on the growth stage. A possible pathway could be deduced using metabolomics analysis, and information regarding physiological characterization and optimal harvesting time was obtained. Highlights: Thirty-one secondary metabolites in C. sinensis leaves were identified. Different period samples were clearly discriminated by PCA plot. Significant changes in the contents of metabolites were revealed during growth.