Sensitive simultaneous determination of three sulfanilamide artificial sweeters by capillary electrophoresis with on-line preconcentration and contactless conductivity detection. (1st December 2015)

• Record Type: Journal Article
• Authors: Yang, Lirong; Zhou, ShengJi; Xiao, Yuezhou; Tang, Yufeng; Xie, Tianyao
• Is Part Of: Food chemistry. Volume 188(2015)
• Page Start: 446
• Page End: 451
• Publication Date: 2015-12-01
• Subjects: Capillary electrophoresis -- Contactless conductivity detection -- On-line preconcentration -- Acesulfame-K -- Sodium saccharin -- Sodium cyclamate; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.04.060
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A sensitive new method for determination of sulfanilamide artificial sweeteners is proposed. The method is based on FASI–CE–C 4 D technique to achieve high sensitivity enhancement and lower LODs. Simplicity, rapidity, low cost, high efficiency and easy sample treatment are some advantages of this method. The method was validated for directly determination of artificial sweeteners in commercial beverages. Abstract: A sensitive method followed by capillary electrophoresis with on-line perconcentration and capacitively coupled contactless conductivity detection (CE–C 4 D) was evaluated as a novel approach for the determination of three sulfanilamide artificial sweeteners (acesulfame-K, sodium saccharin and sodium cyclamate) in beverages. The on-line preconcentration technique, namely field-amplified sample injection, coupled with CE–C 4 D were successfully developed and optimized. The separation was achieved within 10 min under the following conditions: an uncoated fused-silica capillary (45 cm × 50 μm i.d., L eff = 40 cm), 20 mmol L −1 HAc as running buffer, separation voltage of −12 kV, electrokinetic injection of −11 kV × 8 s. The detection limits of acesulfame-K, sodium saccharin and sodium cyclamate were 4.4, 6.7 and 8.8 μg L −1, respectively. The relative standard deviation varied in the range of 3.0–5.0%. Results of this study show a great potential method for the fast screening of these artificial sweeteners contents in commercial beverages.