Identification and quantification of myosin heavy chain isoforms in bovine and porcine longissimus muscles by LC-MS/MS analysis. (March 2017)

• Record Type: Journal Article
• Authors: Kim, Gap-Don; Jeong, Tae-Chul; Cho, Kye Man; Jeong, Jin-Yeon
• Is Part Of: Meat science. Volume 125(2017)
• Page Start: 143
• Page End: 151
• Publication Date: 2017-03
• Subjects: Myosin heavy chain -- LC-MS/MS -- Label-free quantification; Meat -- Periodicals; Meat industry and trade -- Periodicals; Viande -- Périodiques; Viande -- Industrie -- Périodiques; Meat; Meat industry and trade; Periodicals; 641.36
• DOI: 10.1016/j.meatsci.2016.12.002
• Languages: English
• ISSNs: 0309-1740

Abstract

Abstract: To identify and quantify the myosin heavy chain (MHC) isoforms in porcine and bovine longissimus thoracis (LT) muscles, LC-MS/MS and label-free quantification were conducted. 2a, 2x, 2b and slow isoforms were identified in porcine LT muscle, whereas bovine LT muscle contained 2a, 2x and slow isoforms. The highest peak intensity (44.32%) of MHC 2b was observed in porcine LT muscle, whereas the peak intensity of MHC 2a was the highest (71.15%) in bovine LT muscle ( p < 0.05). MHC-based fiber compositions for porcine and bovine LT were also the highest in types IIB (61.13%) and IIA (61.90%), respectively ( p < 0.05). Spectral count and peak intensity had positive correlations with MHC-based fiber composition ( p < 0.01), however the spectral count and peak intensity of total peptides were not acceptable for MHC quantification because of the limited reliability of the protein and common peptide, respectively. Therefore, the peak intensity of unique peptides would be the most acceptable trait for MHC quantification. Highlight: Porcine and bovine myosin heavy chain (MHC) isoforms were identified by LC-MS/MS. Bovine longissimus muscle did not contain MHC 2b. Label-free quantification allows for the quantification of MHCs. Peak intensity (%) of unique peptide is the most acceptable method for MHC quantification.