Response surface methodology to optimise Accelerated Solvent Extraction of steviol glycosides from Stevia rebaudiana Bertoni leaves. (1st January 2015)

• Record Type: Journal Article
• Authors: Jentzer, Jean-Baptiste; Alignan, Marion; Vaca-Garcia, Carlos; Rigal, Luc; Vilarem, Gérard
• Is Part Of: Food chemistry. Volume 166(2015)
• Page Start: 561
• Page End: 567
• Publication Date: 2015-01-01
• Subjects: Stevia rebaudiana Bertoni -- Accelerated Solvent Extraction -- Steviol glycosides -- Genotype screening -- Response surface methodology -- HPLC; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.06.078
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An extraction method of steviol glycosides from stevia leaves was optimised. The extraction method set up is fast and adapted for stevia genotype screening. The experimental results gave a maximal extraction yield. Abstract: Following the approval of steviol glycosides as a food additive in Europe in December 2011, large-scale stevia cultivation will have to be developed within the EU. Thus there is a need to increase the efficiency of stevia evaluation through germplasm enhancement and agronomic improvement programs. To address the need for faster and reproducible sample throughput, conditions for automated extraction of dried stevia leaves using Accelerated Solvent Extraction were optimised. A response surface methodology was used to investigate the influence of three factors: extraction temperature, static time and cycle number on the stevioside and rebaudioside A extraction yields. The model showed that all the factors had an individual influence on the yield. Optimum extraction conditions were set at 100 °C, 4 min and 1 cycle, which yielded 91.8% ± 3.4% of total extractable steviol glycosides analysed. An additional optimisation was achieved by reducing the grind size of the leaves giving a final yield of 100.8% ± 3.3%.