Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas. (13th June 2017)

• Record Type: Journal Article
• Authors: Hu, Shuangfang; Yu, Yigang; Wu, Xinwei; Xia, Xingzhou; Xiao, Xinglong; Wu, Hui
• Is Part Of: International journal of dairy technology. Volume 71:Number 1(2018)
• Page Start: 253
• Page End: 263
• Publication Date: 2017-06-13
• Subjects: Cronobacter -- High‐resolution melting -- Powdered infant formulae -- Pathogen detection -- Pathogen identification; Dairying -- Periodicals; Dairying -- Great Britain -- Periodicals; 637
• DOI: 10.1111/1471-0307.12410
• Languages: English
• ISSNs: 1364-727X

Abstract

Abstract : A combination method of real‐time PCR and high‐resolution melting (HRM) analysis for the rapid detection and specific classification of six pathogenic Cronobacter species based on gene cgcA was developed. HRM profiles with distinct T m (melting temperature) peaks were consistently obtained with each species, represented by a single peak ranging from 86.02 to 86.80 °C. The detection limit ranged from 0.1 to 1 pg per reaction. Desiccated Cronobacter sakazakii and Cronobacter muytjensii of 2 cfu/25 g were successfully detected after 4‐week storage at room temperature. The newly developed method provided a molecular tool for direct detection and simultaneous identification of pathogenic Cronobacter species in powdered infant formulae.