Optimal extraction and fingerprinting of carotenoids by accelerated solvent extraction and liquid chromatography with tandem mass spectrometry. (15th June 2015)

• Record Type: Journal Article
• Authors: Saha, Supradip; Walia, Suresh; Kundu, Aditi; Sharma, Khushbu; Paul, Ranjit Kumar
• Is Part Of: Food chemistry. Volume 177(2015)
• Page Start: 369
• Page End: 375
• Publication Date: 2015-06-15
• Subjects: Accelerated solvent extractor -- Liquid chromatography–mass spectrometry -- Hildebrand solubility parameters -- Carotenoid; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.01.039
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Accelerated solvent extraction is optimized for the extraction of carotenoids from orange carrot. Solvents were selected based on Hildebrand solubility parameters and dielectric constant. Two carotenoids were analyzed and characterized by LC–ESI MS after extraction by ASE. It was concluded that Hildebrand solubility parameter may be applicable for carotenoids. Solvent properties and temperature are the most important affecting the ASE extraction. Abstract: Accelerated solvent extraction (ASE) is applied for the extraction of carotenoids from orange carrot and the extraction parameters were optimized. Two carotenoids, lutein and β-carotene, are selected as the validation process. Hildebrand solubility parameters and dielectric constant of solvents were taken into consideration in selecting solvent mixture. The effects of various experimental parameters, such as temperature, static time, drying agent etc., on the ASE extraction efficiency are investigated systematically. Interactions among the variables were also studied. Furthermore, two carotenoids were analyzed and characterized by LC–ESI MS. The study concluded that Hildebrand solubility parameter approach may be applicable for less polar bioactive molecules like carotenoids. The properties of solvent and extraction temperature are found to be the most important parameters affecting the ASE extraction efficiency of thermolabile natural compounds.